Pharmacological Evaluation of Anti-Inflammatory Activity of Vallarai Chooranam Using Carrageenan-Induced Paw Edema in Wistar Albino Rats

Abstract

The aim of the study was to explore the anti-inflammatory activity of Siddha polyherbal formulation Vallarai Chooranam in albino rats, and to compared with the standard drug indomethacin. The Siddha formulation Vallarai Chooranam is indicated for Azhal Neerchurukku which are comparable to urinary tract infection in the Siddha system of medicine. The current study used a carrageenan-induced paw edema model in Wistar albino rats to assess the anti-inflammatory properties of Vallarai Chooranam. The animals were divided into four groups (n=6): two test groups given VC at doses of 200mg/kg and 400mg/kg of body weight, a standard group treated with indomethacin at 10mg/kg and a disease control group. Acute inflammation was induced by injecting carrageenan, and paw edema was measured hourly for up to five hours. Additionally, histopathological analysis and hematological parameters were carried out. Dunnett's test and one-way ANOVA were used to analyze the data, which were expressed as mean±SEM.A p-value<0.05 was deemed statistically significant. VC administration significantly reduced paw edema, with effects that increased with higher doses; the most effective dose showed results similar to the standard treatment. Histopathological results showed decreased infiltration of inflammatory cells, and hematological parameters were considerably improved. The study's results support the traditional use of Vallarai Chooranam, confirming its strong anti-inflammatory properties.