Evaluation of the Antihypertensive Activity of Seenthil Chooranam in DOCA-Salt-Induced Hypertensive Wistar Albino Rats

Abstract

In recent years, traditional medical formulations are increasingly explored for their antihypertensive potential. Aim: To assess the anti-hypertensive efficacy of Seenthil chooranam and its effects on blood pressure, serum electrolytes and lipid profile in DOCA- salt-induced hypertensive rats. Materials and Methods: Hypertension was induced in rats by subcutaneously administering DOCA salt [10mg/kg, twice weekly] along with 2% sodium chloride supplementation for a period of 43 days. The animals were divided into five groups: normal control, hypertensive control, standard drug group [hydrochlorothiazide 5mg/kg] and two Seenthil chooranam treated groups [200mg/kg and 400mg/kg orally]. Blood pressure was recorded invasively by carotid artery cannulation. Serum sodium, potassium and lipid profile were analysed using standard biochemical methods. Data analysis was carried out using one-way ANOVA followed by Dunnett’s test. Results: DOCA- salt induced rats showed a significant increase in blood pressure, serum sodium, total cholesterol, triglycerides, LDL and VLDL levels, with a decrease in serum potassium and HDL levels. Treatment with Seenthil chooranam reduced the blood pressure, improved electrolyte balance and lipid profile in a dose-dependent manner, with the higher dose showing effects comparable to hydrochlorothiazide. Conclusion: The study concludes that Seenthil chooranam has significant antihypertensive effects in DOCA-salt induced hypertensive rats. The observed improvements in blood pressure, serum electrolytes and lipid profile support the therapeutic potential of Seenthil chooranam in hypertension management. Further investigations are necessary to clarify its mechanism and long-term safety profile.