Evaluation of Ovulation-Inducing Activity of Inji Rasayanam in Female Wistar Albino Rats

Abstract

Polycystic ovarian disease (PCOD) is a common endocrine disorder characterized by anovulation, hyperandrogenism, and hormonal imbalance. Inji Rasayanam, a classical Siddha polyherbal formulation, is traditionally used for reproductive disorders. This study aimed to evaluate the ovulation-inducing activity of Inji Rasayanam in female Wistar albino rats. Twenty-four female Wistar albino rats were divided into four groups (n = 6): normal control (2% CMC), low dose (36mg/kg), high dose (360mg/kg), and standard (clomiphene citrate 10mg/kg). Estrous cycles were synchronized using estradiol and progesterone. The drug was administered orally for 10 days. Serum levels of LH, FSH, estradiol, progesterone, and testosterone were estimated using ELISA. Uterine and ovarian weights were recorded, and histopathological examination of ovarian tissues was performed. Statistical analysis was done using one-way ANOVA followed by Dunnett’s test. Inji Rasayanam significantly increased FSH levels and reduced estrogen and testosterone, with minimal changes in LH and progesterone. Histology showed improved follicular development comparable to the standard drug. Inji Rasayanam may possess ovulation-inducing activity through hormonal modulation and restoration of ovarian architecture.