EFFECT OF MISHREYA ARKA (HYDRO DISTILLATE OF DILL) ON RAT ILEUM, AGAINST ACETYL CHOLINE INDUCED CONTRACTIONS

Abstract

Isolated tissue bath study is a classical and best way to evaluate the effect of any medication on smooth muscles. It allows the tissue to function as a system, it is close to how drug would act in body as whole. Mishreya (Anethum sowa Kurz) or dill is an important herb used as home remedy for common abdominal problems and described in Ayurveda as well as in other systems of medicines also like Unani and modern medicine. Mishreya Arka or hydro-distillate of dill is indicated in indigestion, stomach ache, uterine pain etc. To evaluate the effect of Mishreya Arka on smooth muscles of intestine, the present study has been planned. Mishreya Arka was prepared by following the Ayurvedic formulary of India. Isolated rat ileum was set on organ bath assembly with maintained temperature (37°C), nutrition and oxygenated. Contraction was induced by Acetylcholine. Then response of Mishreya Arka was observed at the dose of 0.5ml, 1ml, 1.5ml and 2ml/ 40ml Tyrode solution. Relaxations or spasmolytic effect was noticed on all the doses of Mishreya Arka against the contraction induced by Acetylcholine. The effective concentration for 50% effect (EC₅₀) of Mishreya Arka was 0.0315 ml/ml. It can be concluded that Mishreya Arka has spasmolytic effect on rat ileum.